Plant Transcription Factor Database
Previous version: v3.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT5G59820.1
Common NameAtZAT12, MMN10.11, RHL41, ZAT12
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family C2H2
Protein Properties Length: 162aa    MW: 17332.1 Da    PI: 10.1879
Description C2H2 family protein
Gene Model
Gene Model ID Type Source Coding Sequence
AT5G59820.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
      zf-C2H2   1 ykCpdCgksFsrksnLkrHirtH 23 
                  ++Cp+Cg  F+    L  H+r+H
                  78********************9 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SuperFamilySSF576671.21E-637104No hitNo description
PfamPF139122.5E-113863IPR007087Zinc finger, C2H2
PROSITE profilePS501578.8913966IPR007087Zinc finger, C2H2
SMARTSM003552.53961IPR015880Zinc finger, C2H2-like
PROSITE patternPS0002804161IPR007087Zinc finger, C2H2
PfamPF139122.4E-1182105IPR007087Zinc finger, C2H2
PROSITE profilePS501579.68182109IPR007087Zinc finger, C2H2
SMARTSM003550.07982104IPR015880Zinc finger, C2H2-like
PROSITE patternPS00028084104IPR007087Zinc finger, C2H2
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0006979Biological Processresponse to oxidative stress
GO:0009408Biological Processresponse to heat
GO:0009611Biological Processresponse to wounding
GO:0009631Biological Processcold acclimation
GO:0009643Biological Processphotosynthetic acclimation
GO:0010200Biological Processresponse to chitin
GO:0010224Biological Processresponse to UV-B
GO:0042538Biological Processhyperosmotic salinity response
GO:0005634Cellular Componentnucleus
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0008270Molecular Functionzinc ion binding
GO:0043565Molecular Functionsequence-specific DNA binding
GO:0044212Molecular Functiontranscription regulatory region DNA binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000013anatomycauline leaf
PO:0000037anatomyshoot apex
PO:0000084anatomyplant sperm cell
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009009anatomyplant embryo
PO:0009025anatomyvascular leaf
PO:0009052anatomyflower pedicel
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0025195anatomypollen tube cell
PO:0001016developmental stageL mature pollen stage
PO:0001054developmental stagevascular leaf senescent stage
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 162 aa     Download sequence    Send to blast
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.242600.0flower| inflorescence| leaf| seed
Expression -- Microarray ? help Back to Top
Source ID E-value
Expression AtlasAT5G59820-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Expressed in roots, stems and flowers. {ECO:0000269|PubMed:9132053}.
Functional Description ? help Back to Top
Source Description
TAIREncodes a zinc finger protein involved in high light and cold acclimation. Overexpression of this putative transcription factor increases the expression level of 9 cold-responsive genes and represses the expression level of 15 cold-responsive genes, including CBF genes. Also, lines overexpressing this gene exhibits a small but reproducible increase in freeze tolerance. Because of the repression of the CBF genes by the overexpression of this gene, the authors speculate that this gene may be involved in negative regulatory circuit of the CBF pathway.
UniProtTranscriptional repressor involved in light acclimation, cold and oxidative stress responses. May regulate a collection of transcripts involved in response to high-light, cold and oxidative stress. {ECO:0000269|PubMed:11069694, ECO:0000269|PubMed:14722088, ECO:0000269|PubMed:15634197, ECO:0000269|PubMed:16183833}.
Function -- GeneRIF ? help Back to Top
  1. Zat12 expression is activated at the transcriptional level during different abiotic stresses and in response to a wound-induced systemic signal
    [PMID: 16183833]
  2. Overall, our findings indicate that H(2) acts as a novel and cytoprotective regulator in coupling ZAT10/12-mediated antioxidant defence and maintenance of ion homeostasis in the improvement of Arabidopsis salt tolerance.
    [PMID: 23185443]
Cis-element ? help Back to Top
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: By H(2)O(2), cold, drought, cold or heat stresses, wounding, cucumber mosaic virus (CMV), exposure to high-intensity light and low-oxygen conditions in roots. {ECO:0000269|PubMed:12368499, ECO:0000269|PubMed:14722088, ECO:0000269|PubMed:15634197, ECO:0000269|PubMed:16183833, ECO:0000269|PubMed:18201973, ECO:0000269|PubMed:18922600}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source Target Gene (A: Activate/R: Repress)
ATRM AT1G07890(A), AT2G30250(A), AT3G46090(A), AT4G25470(R), AT4G25480(R), AT4G25490(R)
Phenotype -- Disruption Phenotype ? help Back to Top
Source Description
UniProtDISRUPTION PHENOTYPE: No visible phenotype under normal growth condition, but enhanced tolerance to heat stress and increased sensitivity to salt stress. {ECO:0000269|PubMed:14722088, ECO:0000269|PubMed:16183833}.
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT5G59820
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAB0154750.0AB015475.1 Arabidopsis thaliana genomic DNA, chromosome 5, P1 clone:MMN10.
GenBankAY0509150.0AY050915.1 Arabidopsis thaliana putative zinc finger protein Zat12 (At5g59820) mRNA, complete cds.
GenBankAY1338180.0AY133818.1 Arabidopsis thaliana clone U11346 putative zinc finger protein Zat12 (At5g59820) mRNA, complete cds.
GenBankCP0026880.0CP002688.1 Arabidopsis thaliana chromosome 5 sequence.
GenBankX986730.0X98673.1 A.thaliana mRNA for ZAT12 protein.
GenBankX986740.0X98674.1 A.thaliana zat12 gene.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_200790.11e-117high light responsive zinc finger protein ZAT12
SwissprotQ424101e-119ZAT12_ARATH; Zinc finger protein ZAT12
TrEMBLD7MT321e-111D7MT32_ARALL; Putative uncharacterized protein
STRINGAT5G59820.11e-117(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
Representative plantOGRP13115149
Publications ? help Back to Top
  1. Iida A,Kazuoka T,Torikai S,Kikuchi H,Oeda K
    A zinc finger protein RHL41 mediates the light acclimatization response in Arabidopsis.
    Plant J., 2000. 24(2): p. 191-203
  2. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
  3. Klok EJ, et al.
    Expression profile analysis of the low-oxygen response in Arabidopsis root cultures.
    Plant Cell, 2002. 14(10): p. 2481-94
  4. Yamada K, et al.
    Empirical analysis of transcriptional activity in the Arabidopsis genome.
    Science, 2003. 302(5646): p. 842-6
  5. Rizhsky L,Davletova S,Liang H,Mittler R
    The zinc finger protein Zat12 is required for cytosolic ascorbate peroxidase 1 expression during oxidative stress in Arabidopsis.
    J. Biol. Chem., 2004. 279(12): p. 11736-43
  6. Zhang JZ,Creelman RA,Zhu JK
    From laboratory to field. Using information from Arabidopsis to engineer salt, cold, and drought tolerance in crops.
    Plant Physiol., 2004. 135(2): p. 615-21
  7. Vogel JT,Zarka DG,Van Buskirk HA,Fowler SG,Thomashow MF
    Roles of the CBF2 and ZAT12 transcription factors in configuring the low temperature transcriptome of Arabidopsis.
    Plant J., 2005. 41(2): p. 195-211
  8. Fowler SG,Cook D,Thomashow MF
    Low temperature induction of Arabidopsis CBF1, 2, and 3 is gated by the circadian clock.
    Plant Physiol., 2005. 137(3): p. 961-8
  9. Ramonell K, et al.
    Loss-of-function mutations in chitin responsive genes show increased susceptibility to the powdery mildew pathogen Erysiphe cichoracearum.
    Plant Physiol., 2005. 138(2): p. 1027-36
  10. Davletova S,Schlauch K,Coutu J,Mittler R
    The zinc-finger protein Zat12 plays a central role in reactive oxygen and abiotic stress signaling in Arabidopsis.
    Plant Physiol., 2005. 139(2): p. 847-56
  11. Skinner JS, et al.
    Mapping of barley homologs to genes that regulate low temperature tolerance in Arabidopsis.
    Theor. Appl. Genet., 2006. 112(5): p. 832-42
  12. Miller G,Mittler R
    Could heat shock transcription factors function as hydrogen peroxide sensors in plants?
    Ann. Bot., 2006. 98(2): p. 279-88
  13. Libault M,Wan J,Czechowski T,Udvardi M,Stacey G
    Identification of 118 Arabidopsis transcription factor and 30 ubiquitin-ligase genes responding to chitin, a plant-defense elicitor.
    Mol. Plant Microbe Interact., 2007. 20(8): p. 900-11
  14. Suzuki N,Bajad S,Shuman J,Shulaev V,Mittler R
    The transcriptional co-activator MBF1c is a key regulator of thermotolerance in Arabidopsis thaliana.
    J. Biol. Chem., 2008. 283(14): p. 9269-75
  15. Safrany J, et al.
    Identification of a novel cis-regulatory element for UV-B-induced transcription in Arabidopsis.
    Plant J., 2008. 54(3): p. 402-14
  16. Ascencio-Ib
    Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection.
    Plant Physiol., 2008. 148(1): p. 436-54
  17. Wang Y, et al.
    Transcriptome analyses show changes in gene expression to accompany pollen germination and tube growth in Arabidopsis.
    Plant Physiol., 2008. 148(3): p. 1201-11
  18. Mitsuya Y, et al.
    Spermine signaling plays a significant role in the defense response of Arabidopsis thaliana to cucumber mosaic virus.
    J. Plant Physiol., 2009. 166(6): p. 626-43
  19. Doherty CJ,Van Buskirk HA,Myers SJ,Thomashow MF
    Roles for Arabidopsis CAMTA transcription factors in cold-regulated gene expression and freezing tolerance.
    Plant Cell, 2009. 21(3): p. 972-84
  20. Dong CJ,Liu JY
    The Arabidopsis EAR-motif-containing protein RAP2.1 functions as an active transcriptional repressor to keep stress responses under tight control.
    BMC Plant Biol., 2010. 10: p. 47
  21. Cantrel C, et al.
    Nitric oxide participates in cold-responsive phosphosphingolipid formation and gene expression in Arabidopsis thaliana.
    New Phytol., 2011. 189(2): p. 415-27
  22. Takahashi F,Mizoguchi T,Yoshida R,Ichimura K,Shinozaki K
    Calmodulin-dependent activation of MAP kinase for ROS homeostasis in Arabidopsis.
    Mol. Cell, 2011. 41(6): p. 649-60
  23. Qin Y, et al.
    Over-expression of TaMYB33 encoding a novel wheat MYB transcription factor increases salt and drought tolerance in Arabidopsis.
    Mol. Biol. Rep., 2012. 39(6): p. 7183-92
  24. Mehterov N, et al.
    Oxidative stress provokes distinct transcriptional responses in the stress-tolerant atr7 and stress-sensitive loh2 Arabidopsis thaliana mutants as revealed by multi-parallel quantitative real-time PCR analysis of ROS marker and antioxidant genes.
    Plant Physiol. Biochem., 2012. 59: p. 20-9
  25. Xie Y,Mao Y,Lai D,Zhang W,Shen W
    H(2) enhances arabidopsis salt tolerance by manipulating ZAT10/12-mediated antioxidant defence and controlling sodium exclusion.
    PLoS ONE, 2012. 7(11): p. e49800
  26. Schweizer F,Bodenhausen N,Lassueur S,Masclaux FG,Reymond P
    Differential Contribution of Transcription Factors to Arabidopsis thaliana Defense Against Spodoptera littoralis.
    Front Plant Sci, 2013. 4: p. 13
  27. Hahn A, et al.
    Plant core environmental stress response genes are systemically coordinated during abiotic stresses.
    Int J Mol Sci, 2013. 14(4): p. 7617-41
  28. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73
  29. Meissner R,Michael AJ
    Isolation and characterisation of a diverse family of Arabidopsis two and three-fingered C2H2 zinc finger protein genes and cDNAs.
    Plant Mol. Biol., 1997. 33(4): p. 615-24